rat monoclonal anti cd105  (R&D Systems)

Journal: Theranostics

Article Title: Exosome-mediated delivery of an anti-angiogenic peptide inhibits pathological retinal angiogenesis

doi: 10.7150/thno.54755

Figure Lengend Snippet: EXO KV11 suppresses VEGF-induced vascular leakage in vivo . (A) Schematic depiction of the pre-treatment procedure in a VEGF-induced vascular leakage model. EXO KV11 , KV11, EXO, or vehicle was retro-orbitally injected in adult wild type C57BL/6J mice. After 24 h, 100 ng VEGF was intravitreally injected to induce vascular leakage in the retina. The mice were sacrificed for analysis after another 24 h. (B) FITC-dextran was injected and the retinas were harvested at the endpoint. Representative images of flat-mounted retina show extravasated FITC-dextran and CD105 + vessels. (C) Quantification of dextran leakage in (B) (n = 7 - 10 mice per condition). (D) Representative confocal images of anti-CD105, anti-Claudin-5-stained retinal vessels in mice treated as in (A). (E) Representative images of F4/80 + macrophages (green) and CD105 + vessels in retinas treated as in (A). (F) Quantification of macrophage infiltration in (E) (n = 4 - 9 mice per condition). (G) Representative photographs of Evans blue leakage in SD rats ears in an auricular Miles assay with intradermal injection with VEGF, VEGF+KV11 mixture, or VEGF+EXO KV11 mixture. The data represent as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA followed by Tukey's multiple comparisons test in (C), (F). Scale bars, 50 µm.

Article Snippet: For IF staining of red blood cells, blood vessels, macrophage and microglia, flat-mounted retinas were incubated in blocking solution with following primary antibodies at 4 °C overnight: rat anti-TER119 monoclonal (1:200, MAB1125, RD, US); rat anti-CD105 monoclonal (1:200, MAB1320, RD, US); rabbit anti-F4/80 monoclonal (1:200, 30325S, Cell Signaling Technology, US); mouse anti-Claudin-5 monoclonal (1:200, 35-2500, Invitrogen, US) and rabbit anti-ZO-1 polyclonal (1:300, 61-7300, Invitrogen, US).

Techniques: In Vivo, Injection, Staining

Journal: Theranostics

Article Title: Exosome-mediated delivery of an anti-angiogenic peptide inhibits pathological retinal angiogenesis

doi: 10.7150/thno.54755

Figure Lengend Snippet: EXO KV11 has therapeutic effect in post-treatment model. (A) Schematic depiction of the post-treatment procedure in VEGF-induced vascular leakage model. 100 ng VEGF was intravitreally injected to induce vascular leakage in the retina. After 24 h, EXO KV11 , KV11, EXO, or vehicle was retro-orbitally injected in adult wild type C57BL/6J mice. The mice were sacrificed for analysis after another 48 h. (B) FITC-dextran was injected and the retinas were harvested after treatment. Representative images of flat-mounted retina show extravasated FITC-dextran and CD105 + vessels. (C) Quantification of dextran leakage in (B) (n = 4 - 6 mice per condition). (D) Representative images of F4/80 + macrophages (green) and CD105 + vessels in retinas treated as in (A). (E) Quantification of macrophage infiltration in (D) (n = 4 - 6 mice per condition). The data represent as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA followed by Tukey's multiple comparisons test in (C), (E). Scale bars, 50 µm.

Article Snippet: For IF staining of red blood cells, blood vessels, macrophage and microglia, flat-mounted retinas were incubated in blocking solution with following primary antibodies at 4 °C overnight: rat anti-TER119 monoclonal (1:200, MAB1125, RD, US); rat anti-CD105 monoclonal (1:200, MAB1320, RD, US); rabbit anti-F4/80 monoclonal (1:200, 30325S, Cell Signaling Technology, US); mouse anti-Claudin-5 monoclonal (1:200, 35-2500, Invitrogen, US) and rabbit anti-ZO-1 polyclonal (1:300, 61-7300, Invitrogen, US).

Techniques: Injection

Journal: Theranostics

Article Title: Exosome-mediated delivery of an anti-angiogenic peptide inhibits pathological retinal angiogenesis

doi: 10.7150/thno.54755

Figure Lengend Snippet: EXO KV11 suppresses VEGF-induced vascular leakage in vivo . (A) Schematic depiction of the pre-treatment procedure in a VEGF-induced vascular leakage model. EXO KV11 , KV11, EXO, or vehicle was retro-orbitally injected in adult wild type C57BL/6J mice. After 24 h, 100 ng VEGF was intravitreally injected to induce vascular leakage in the retina. The mice were sacrificed for analysis after another 24 h. (B) FITC-dextran was injected and the retinas were harvested at the endpoint. Representative images of flat-mounted retina show extravasated FITC-dextran and CD105 + vessels. (C) Quantification of dextran leakage in (B) (n = 7 - 10 mice per condition). (D) Representative confocal images of anti-CD105, anti-Claudin-5-stained retinal vessels in mice treated as in (A). (E) Representative images of F4/80 + macrophages (green) and CD105 + vessels in retinas treated as in (A). (F) Quantification of macrophage infiltration in (E) (n = 4 - 9 mice per condition). (G) Representative photographs of Evans blue leakage in SD rats ears in an auricular Miles assay with intradermal injection with VEGF, VEGF+KV11 mixture, or VEGF+EXO KV11 mixture. The data represent as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA followed by Tukey's multiple comparisons test in (C), (F). Scale bars, 50 µm.

Article Snippet: In VEGF induced retina vascular leakage model, retinas were incubated in blocking solution with the primary antibody rat anti-CD105 monoclonal (1:200, MAB1320, RD, US) at 4 °C overnight after permeabilization and blocking.

Techniques: In Vivo, Injection, Staining

Journal: Theranostics

Article Title: Exosome-mediated delivery of an anti-angiogenic peptide inhibits pathological retinal angiogenesis

doi: 10.7150/thno.54755

Figure Lengend Snippet: EXO KV11 has therapeutic effect in post-treatment model. (A) Schematic depiction of the post-treatment procedure in VEGF-induced vascular leakage model. 100 ng VEGF was intravitreally injected to induce vascular leakage in the retina. After 24 h, EXO KV11 , KV11, EXO, or vehicle was retro-orbitally injected in adult wild type C57BL/6J mice. The mice were sacrificed for analysis after another 48 h. (B) FITC-dextran was injected and the retinas were harvested after treatment. Representative images of flat-mounted retina show extravasated FITC-dextran and CD105 + vessels. (C) Quantification of dextran leakage in (B) (n = 4 - 6 mice per condition). (D) Representative images of F4/80 + macrophages (green) and CD105 + vessels in retinas treated as in (A). (E) Quantification of macrophage infiltration in (D) (n = 4 - 6 mice per condition). The data represent as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA followed by Tukey's multiple comparisons test in (C), (E). Scale bars, 50 µm.

Article Snippet: In VEGF induced retina vascular leakage model, retinas were incubated in blocking solution with the primary antibody rat anti-CD105 monoclonal (1:200, MAB1320, RD, US) at 4 °C overnight after permeabilization and blocking.

Techniques: Injection

Journal: Cell Reports

Article Title: Skeletal progenitors preserve proliferation and self-renewal upon inhibition of mitochondrial respiration by rerouting the TCA cycle

doi: 10.1016/j.celrep.2022.111105

Figure Lengend Snippet:

Article Snippet: Rat monoclonal anti-CD105 (Endoglin), PE , eBioscience , Cat# 12-1051-81, RRID: AB_657526.

Techniques: Recombinant, Protease Inhibitor, Reverse Transcription, Bicinchoninic Acid Protein Assay, Isolation, Methylation, Quantitative RT-PCR, Plasmid Preparation, Control, shRNA, Software, Membrane, Western Blot

Journal: Cell and Tissue Research

Article Title: Hypoxia pretreatment enhances the therapeutic potential of mesenchymal stem cells (BMSCs) on ozone-induced lung injury in rats

doi: 10.1007/s00441-022-03627-8

Figure Lengend Snippet: a – d Flow-cytometric analysis of the cell surface markers showing MSCs that express CD105 (mesenchymal stem cell surface marker) and do not express CD34 (hematopoietic stem cell marker). e PKH26-labeled stem cells appearing as bright red dots (arrow) in MSC-treated group. f PKH26-labeled stem cells appearing as bright red dots (arrow) in hypoxia-pretreated MSCs group indicating increased stem cells homing (fluorescent microscope × 200, scale bar 50 μm)

Article Snippet: For CD105 detection, sections were incubated with rat anti-mouse CD105 monoclonal antibody, eBioscience (Cat. No. 14–1051-82, 1:200 dilution; Thermo Fisher Scientific, Rockford, USA).

Techniques: Marker, Labeling, Microscopy

Journal: Cell and Tissue Research

Article Title: Hypoxia pretreatment enhances the therapeutic potential of mesenchymal stem cells (BMSCs) on ozone-induced lung injury in rats

doi: 10.1007/s00441-022-03627-8

Figure Lengend Snippet: Immunohistochemical staining for CD105-positive stem cells showing negative results in control and ozone-treated groups ( a and b , respectively), and positive immunostaining for CD105 in MSC-treated groups with more homing in hypoxia-pretreated MSC group (d) than normoxic MSC group (c)

Article Snippet: For CD105 detection, sections were incubated with rat anti-mouse CD105 monoclonal antibody, eBioscience (Cat. No. 14–1051-82, 1:200 dilution; Thermo Fisher Scientific, Rockford, USA).

Techniques: Immunohistochemical staining, Staining, Control, Immunostaining

Journal: Stem Cell Research & Therapy

Article Title: Amniotic fluid stem cells ameliorate cisplatin-induced acute renal failure through induction of autophagy and inhibition of apoptosis

doi: 10.1186/s13287-019-1476-6

Figure Lengend Snippet: List of antibodies with their dilutions, sources, and catalog numbers

Article Snippet: Rat monoclonal CD105 ( Cat# sc-71042) , 1:100 (FCM) , Santa Cruz Biotechnology, CA, USA.

Techniques: Plasmid Preparation

Journal: Stem Cell Research & Therapy

Article Title: Amniotic fluid stem cells ameliorate cisplatin-induced acute renal failure through induction of autophagy and inhibition of apoptosis

doi: 10.1186/s13287-019-1476-6

Figure Lengend Snippet: Morphology and phenotypic characterization of AFSC. a Representative photomicrographs of amniotic fluid stem cells (AFSC) in culture showing spindle-shaped morphology in passage 3 (scale bar: 100 μm). b Phenotypic characterization of AFSC by flow cytometry showing the expression of cell-surface markers, viz, CD73, CD90, CD105, MHC Class II, and CD45, and intracellular renal progenitor markers, viz, WT1, SIX2, and PAX2 (green or red lines detected with FITC- and PE-conjugated antibodies, respectively, and black lines represent isotype controls). PE, phycoerythrin; FITC, fluorescein isothiocyanate

Article Snippet: Rat monoclonal CD105 ( Cat# sc-71042) , 1:100 (FCM) , Santa Cruz Biotechnology, CA, USA.

Techniques: Flow Cytometry, Expressing